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c57bl6n mice (Charles River Laboratories)

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Charles River Laboratories c57bl6n mice
C57bl6n Mice, supplied by Charles River Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c57bl6n+mice/balb+c+mice/pm41951829-39-27-31
Average 86 stars, based on 1 article reviews

c57bl6n mice - by Bioz Stars, 2026-09

86/100 stars

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Article Title: Intravenous SARS-CoV-2 Spike protein induces neuroinflammation and alpha-synuclein accumulation in brain regions relevant to Parkinson's disease.
Article Snippet: 4 121 2.2.Animal experiments 122 Male and female C57BL6N mice were purchased from Charles River (027C57BL/6).

Article Title: MitoSNO inhibits mitochondrial hydrogen peroxide generation by α-ketoglutarate dehydrogenase
Article Snippet: Male C57BL6N mice were purchased from Charles River at 9 to 10 weeks and were maintained standard maintenance diet (Inotiv TD2020X) at 21 °C on a 12-h light/dark cycle until 11 to 12 weeks of age.

Article Title: Upregulated FoxO1 promotes arrhythmogenesis in mice with heart failure and preserved ejection fraction.
Article Snippet: Male C57BL6N mice (8–12week-old, Charles River Laboratories, MA) were used.

Article Title: Accumulation of succinate in the blood is a potential early indicator of metabolic dysfunction-associated steatotic liver disease (MASLD).
Article Snippet: There is currently a need to identify new biomarkers to diagnose metabolic dysfunction-associated steatotic liver disease (MASLD).. Here, using C57BL6N male mice fed a high-fat diet (HFD), we provide evidence that extracellular succinate buildup is a sex-dependent diagnostic marker for MASLD.. Male mice fed the HFD for 2weeks developed simple steatosis, which was associated with the plasma buildup of succinate to 50 μM.

Article Title: In vivo armed macrophages curb liver metastasis through tumor-reactive T-cell rejuvenation
Article Snippet: In this study we used C57BL6n mice obtained from Charles River Laboratories, Italy.

Control:

Article Title: Ethanol induction of FGF21 in the liver is dependent on histone acetylation and ligand activation of ChREBP by glycerol-3-phosphate
Article Snippet: .. Global Adh1 -KO ( ) and Aldh2 -KO ( ) mice were backcrossed to C57BL6N background for more than 10 generations, and corresponding control C57BL6N mice were purchased from Charles River Laboratory. ..

Article Title: Ethanol induction of FGF21 in the liver is dependent on histone acetylation and ligand activation of ChREBP by glycerol-3-phosphate.
Article Snippet: .. Global Adh1- KO (28) and Aldh2- KO (29) mice were backcrossed to C57BL6N background for more than 10 generations, and corresponding control C57BL6N mice were purchased from Charles River Laboratory. ..

Generated:

Article Title: Visual attention is linked to temporally structured noradrenaline release in the medial prefrontal cortex.
Article Snippet: .. Male adult TH-Cre+/- C57BL/6 J mice were generated by Dr. Ted Dawson at Johns Hopkins University [46], and were maintained heterozygous by breeding in house with female C57Bl6N mice, supplied from Charles River. ..

Image Search Results

In vivo comparison of SM102-LNP and SM102–CDL9-LNP. (A) Representative images of mice injected with 4 μg of Nluc mRNA formulated with LNPs via subcutaneous route of administration. (B) Total flux (p s −1 ) of mice injected with 4 μg of mRNA encoding Nluc formulated with LNPs for subcutaneous route (mean + SE of N = 3–4). (C) Antigen-specific antibody levels in the blood. Anti-OVA IgG1 titers were measured by ELISA (mean + SE of N = 5, * p < 0.05). (D) Prophylactic anti-tumor effect: C57BL6N mice were treated with PBS, OVA-encoding naked mRNA, OVA-encoded mRNA formulated with SM102-LNP or SM102/CDL9-LNP at 7 and 14 days before tumor inoculation. The mice were inoculated with E.G7-OVA cells and tumor volume was monitored. The plots represent the mean + SE (total of 5 mice/group) (#: * p < 0.05 day 8, 15–23, SM102 or SM102/CDL9 vs. PBS or naked mRNA, ** p < 0.01 day 13, SM102 or SM102/CDL9 vs. PBS or naked mRNA). (E) Representative dot plots for detecting splenic antigen-specific cytotoxic CD8+ T cells. The gating shown in light blue in the dot plot indicates OVA tetramer-positive cells within the CD8-positive population. (F) The percentage of splenic antigen-specific CD8+ T cells (mean + SE of N = 5).

Journal: RSC Medicinal Chemistry

Article Title: In vivo demonstration of enhanced mRNA delivery by cyclic disulfide-containing lipid nanoparticles for facilitating endosomal escape †

doi: 10.1039/d5md00084j

Figure Lengend Snippet: In vivo comparison of SM102-LNP and SM102–CDL9-LNP. (A) Representative images of mice injected with 4 μg of Nluc mRNA formulated with LNPs via subcutaneous route of administration. (B) Total flux (p s −1 ) of mice injected with 4 μg of mRNA encoding Nluc formulated with LNPs for subcutaneous route (mean + SE of N = 3–4). (C) Antigen-specific antibody levels in the blood. Anti-OVA IgG1 titers were measured by ELISA (mean + SE of N = 5, * p < 0.05). (D) Prophylactic anti-tumor effect: C57BL6N mice were treated with PBS, OVA-encoding naked mRNA, OVA-encoded mRNA formulated with SM102-LNP or SM102/CDL9-LNP at 7 and 14 days before tumor inoculation. The mice were inoculated with E.G7-OVA cells and tumor volume was monitored. The plots represent the mean + SE (total of 5 mice/group) (#: * p < 0.05 day 8, 15–23, SM102 or SM102/CDL9 vs. PBS or naked mRNA, ** p < 0.01 day 13, SM102 or SM102/CDL9 vs. PBS or naked mRNA). (E) Representative dot plots for detecting splenic antigen-specific cytotoxic CD8+ T cells. The gating shown in light blue in the dot plot indicates OVA tetramer-positive cells within the CD8-positive population. (F) The percentage of splenic antigen-specific CD8+ T cells (mean + SE of N = 5).

Article Snippet: 4-Week-old female ICR mice and 4-week-old female C57BL6N mice were purchased from Japan SLC.

Techniques: In Vivo, Comparison, Injection, Enzyme-linked Immunosorbent Assay

MitoSNO mitigates the hypergeneration of mtH 2 O 2 by KGDH and rescues OxPhos in liver mitochondria isolated from mice fed a high-fat diet. A , total body mass of male mice fed a control-matched diet (CD; open circles ) or high-fat diet (HFD; filled in squares ) from 5 to 13 weeks of age. N = 4, mean ± SD, two-way ANOVA with a post hoc Tukey’s test. B , abdominal and liver mass of male mice fed a CD or HFD. a = the value is significantly different when HFD is compared to CD at different time points. N = 4, mean ± SD, paired two-tailed Student t test. C , H&E and Oil Red O stains for liver sections collected from male mice fed a CD or HFD. a = the value is significantly different when compared to CD. N = 4. D , plasma succinate levels. N = 4, mean ± SD, paired two-tailed Student t test. E , rate of mtH 2 O 2 production by liver mitochondria collected from mice fed a CD or HFD. Liver mitochondria were incubated in buffer alone, MitoSNO (500 nM), MitoNAP (500 nM), KMV (10 mM), or valproic acid (VA, 10 mM) prior to measuring mtH 2 O 2 generation. Pyruvate and malate served as substrates. N = 4, mean ± SD, two-way ANOVA with a post hoc Tukey’s test. a = the value is significantly different from the control in the CD group. b = the value is significantly different from the control in the HFD group. c = the value for the MitoNAP group is significantly different when compared to all other groups. ◦ ( white bar ) = −S1 and S3 ▪ ( gray bar ) = +S1 and S3. F , measurement of the impact of a CD and HFD on the different states of respiration and the impact of MitoSNO on OxPhos. After assessing state 4 respiration, MitoSNO (500 nM), MitoNAP (500 nM), KMV (10 mM), or valproic acid (VA, 10 mM) were injected into the wells. The impact of all four compounds on state 4, state 3, and state 4 O respiration was tested. In the figure panels: A : injection of MitoSNO, MitoNAP, KMV, or valproic acid, B : injection of ADP, C : injection of oligomycin, D : injection of antimycin A. Values for state 3 and state 4 (after injection of port A contents) were used to calculate the RCR. N = 4, mean ± SD, 2-way ANOVA with a post hoc Tukey’s test. a = the value is significantly different from the control in the CD group. b = the value is significantly different from the control in the HFD group. c = the value for the CD is significantly different from the HFD for individual experimental treatments. ◦ ( white bar ) = −S1 and S3 ▪ ( gray bar ) = +S1 and S3. KGDH, α-ketoglutarate dehydrogenase; KMV, 2-keto-3-methylvaleric acid; MitoNAP, mitochondria-selective N-acetyl-penicillamine; MitoSNO, mitochondria-targeted S-nitrosating agent; mtH 2 O 2 , mitochondrial hydrogen peroxide; OxPhos, oxidative phosphorylation; RCR, respiratory control ratio.

Journal: The Journal of Biological Chemistry

Article Title: MitoSNO inhibits mitochondrial hydrogen peroxide generation by α-ketoglutarate dehydrogenase

doi: 10.1016/j.jbc.2025.108510

Figure Lengend Snippet: MitoSNO mitigates the hypergeneration of mtH 2 O 2 by KGDH and rescues OxPhos in liver mitochondria isolated from mice fed a high-fat diet. A , total body mass of male mice fed a control-matched diet (CD; open circles ) or high-fat diet (HFD; filled in squares ) from 5 to 13 weeks of age. N = 4, mean ± SD, two-way ANOVA with a post hoc Tukey’s test. B , abdominal and liver mass of male mice fed a CD or HFD. a = the value is significantly different when HFD is compared to CD at different time points. N = 4, mean ± SD, paired two-tailed Student t test. C , H&E and Oil Red O stains for liver sections collected from male mice fed a CD or HFD. a = the value is significantly different when compared to CD. N = 4. D , plasma succinate levels. N = 4, mean ± SD, paired two-tailed Student t test. E , rate of mtH 2 O 2 production by liver mitochondria collected from mice fed a CD or HFD. Liver mitochondria were incubated in buffer alone, MitoSNO (500 nM), MitoNAP (500 nM), KMV (10 mM), or valproic acid (VA, 10 mM) prior to measuring mtH 2 O 2 generation. Pyruvate and malate served as substrates. N = 4, mean ± SD, two-way ANOVA with a post hoc Tukey’s test. a = the value is significantly different from the control in the CD group. b = the value is significantly different from the control in the HFD group. c = the value for the MitoNAP group is significantly different when compared to all other groups. ◦ ( white bar ) = −S1 and S3 ▪ ( gray bar ) = +S1 and S3. F , measurement of the impact of a CD and HFD on the different states of respiration and the impact of MitoSNO on OxPhos. After assessing state 4 respiration, MitoSNO (500 nM), MitoNAP (500 nM), KMV (10 mM), or valproic acid (VA, 10 mM) were injected into the wells. The impact of all four compounds on state 4, state 3, and state 4 O respiration was tested. In the figure panels: A : injection of MitoSNO, MitoNAP, KMV, or valproic acid, B : injection of ADP, C : injection of oligomycin, D : injection of antimycin A. Values for state 3 and state 4 (after injection of port A contents) were used to calculate the RCR. N = 4, mean ± SD, 2-way ANOVA with a post hoc Tukey’s test. a = the value is significantly different from the control in the CD group. b = the value is significantly different from the control in the HFD group. c = the value for the CD is significantly different from the HFD for individual experimental treatments. ◦ ( white bar ) = −S1 and S3 ▪ ( gray bar ) = +S1 and S3. KGDH, α-ketoglutarate dehydrogenase; KMV, 2-keto-3-methylvaleric acid; MitoNAP, mitochondria-selective N-acetyl-penicillamine; MitoSNO, mitochondria-targeted S-nitrosating agent; mtH 2 O 2 , mitochondrial hydrogen peroxide; OxPhos, oxidative phosphorylation; RCR, respiratory control ratio.

Article Snippet: For experiments where mice were fed to an HFD, male C57BL6N mice were purchased from Charles River at 4 weeks of age, fed the TD2020X diet for 1 week and then placed on a were placed on an HFD (Inotiv TD.06415) or matched low-fat CD (Inotiv TD.06416) until 12 weeks of age.

Techniques: Isolation, Control, Two Tailed Test, Clinical Proteomics, Incubation, Injection, Phospho-proteomics